PrimerProspector： 用于设计和分类分析条形码聚合酶链反应引物的从头设计
PrimerProspector: de novo design and taxonomic analysis of barcoded polymerase chain reaction primers.
机构信息
Department of Molecular, Cellular, and Developmental Biology, Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, CO 80309, USA.
出版信息
Bioinformatics. 2011 Apr 15;27(8):1159-61. doi: 10.1093/bioinformatics/btr087. Epub 2011 Feb 23.
MOTIVATION
PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task.
RESULTS
PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data.
AVAILABILITY
PrimerProspector is open-source software available at http://pprospector.sourceforge.net
CONTACT
SUPPLEMENTARY INFORMATION
Supplementary data are available at Bioinformatics online.
动机
聚合酶链式反应（PCR）扩增 DNA 是高通量测序技术的许多应用中的关键初步步骤，但新型条形码引物的设计和新型或现有引物的分类分析仍然是一项具有挑战性的任务。
结果
PrimerProspector 是一个开源软件包，允许研究人员从序列集合中开发新的引物，并根据分类学数据评估现有引物。
可用性
PrimerProspector 是一个开源软件，可在 http://pprospector.sourceforge.net 获得。
联系人
补充信息
补充数据可在 Bioinformatics 在线获得。
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