Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine WA, Nedlands, WA, Australia. Lyn.O'
J Microbiol Methods. 2011 May;85(2):173-4. doi: 10.1016/j.mimet.2011.02.014. Epub 2011 Mar 3.
The DNA of some bacteria is broken up by Tris-dependent endonuclease activity during the process of sample preparation for pulsed field gel electrophoresis (PFGE). Adding thiourea to the electophoresis buffer for isolates that exhibit DNA degradation has been the method used for many bacterial genera. For a particular group of isolates of Serratia marcescens this method was unsuccessful. A combination of techniques was used to overcome the problem.
在用于脉冲场凝胶电泳 (PFGE) 的样品制备过程中，某些细菌的 DNA 会被 Tris 依赖性内切酶活性打断。对于表现出 DNA 降解的分离株，在电泳缓冲液中添加硫脲一直是许多细菌属使用的方法。对于一组特定的粘质沙雷氏菌分离株，这种方法并不成功。使用了多种技术来克服这个问题。
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