杜洛克×皮特兰杂交群体中与腿部无力相关性状的数量性状位点分析。
Quantitative trait loci analysis for leg weakness-related traits in a Duroc × Pietrain crossbred population.
机构信息
Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany.
出版信息
Genet Sel Evol. 2011 Mar 20;43(1):13. doi: 10.1186/1297-9686-43-13.
BACKGROUND
Leg weakness issues are a great concern for the pig breeding industry, especially with regard to animal welfare. Traits associated with leg weakness are partly influenced by the genetic background of the animals but the genetic basis of these traits is not yet fully understood. The aim of this study was to identify quantitative trait loci (QTL) affecting leg weakness in pigs.
METHODS
Three hundred and ten F₂ pigs from a Duroc × Pietrain resource population were genotyped using 82 genetic markers. Front and rear legs and feet scores were based on the standard scoring system. Osteochondrosis lesions were examined histologically at the head and the condylus medialis of the left femur and humerus. Bone mineral density, bone mineral content and bone mineral area were measured in the whole ulna and radius bones using dual energy X-ray absorptiometry. A line-cross model was applied to determine QTL regions associated with leg weakness using the QTL Express software.
RESULTS
Eleven QTL affecting leg weakness were identified on eight autosomes. All QTL reached the 5% chromosome-wide significance level. Three QTL were associated with osteochondrosis on the humerus end, two with the fore feet score and two with the rear leg score. QTL on SSC2 and SSC3 influencing bone mineral content and bone mineral density, respectively, reached the 5% genome-wide significance level.
CONCLUSIONS
Our results confirm previous studies and provide information on new QTL associated with leg weakness in pigs. These results contribute towards a better understanding of the genetic background of leg weakness in pigs.
背景
腿部虚弱问题是养猪业非常关注的问题，尤其是在动物福利方面。与腿部虚弱相关的特征部分受动物遗传背景的影响，但这些特征的遗传基础尚未完全了解。本研究旨在确定影响猪腿部虚弱的数量性状位点（QTL）。
方法
使用 82 个遗传标记对来自杜洛克×皮特兰资源群体的 310 头 F₂猪进行了基因分型。根据标准评分系统对前腿和后腿以及脚部进行评分。在左侧股骨和肱骨的头部和内侧髁状突处通过组织学检查骨软骨病病变。使用双能 X 射线吸收仪测量整个尺骨和桡骨的骨矿物质密度、骨矿物质含量和骨矿物质面积。使用 QTL Express 软件通过线交叉模型确定与腿部虚弱相关的 QTL 区域。
结果
在 8 条常染色体上确定了 11 个影响腿部虚弱的 QTL。所有 QTL 均达到了 5%染色体-wide 显著水平。3 个 QTL 与肱骨末端的骨软骨病有关，2 个与前脚评分有关，2 个与后腿评分有关。影响 SSC2 和 SSC3 上骨矿物质含量和骨矿物质密度的 QTL 分别达到了 5%基因组-wide 显著水平。
结论
我们的结果证实了先前的研究，并提供了与猪腿部虚弱相关的新 QTL 的信息。这些结果有助于更好地了解猪腿部虚弱的遗传背景。
Zotero 插件