鼻腔黏膜和分泌物中抗菌肽的差异表达模式。

Differential expression pattern of antimicrobial peptides in nasal mucosa and secretion.

机构信息

Department of Otorhinolaryngology, Head and Neck Surgery, University of Kiel, Kiel, Germany.

出版信息

Rhinology. 2011 Mar;49(1):107-11. doi: 10.4193/Rhino10.036.

DOI:10.4193/Rhino10.036

PMID:21468384

Abstract

OBJECTIVE

The intact nasal barrier is a prerequisite for a functioning defense of the upper airway system, in particular the permanent threat by inhaled potentially harmful microorganisms. Antimicrobial peptides (AMP) play an important role in maintaining barrier function. There is few data about AMP in respect of nasal mucosa. This study is addressed to gain further insight into the differential AMP expression and secretion pattern according to defined anatomical regions of the vestibulum nasi and turbinates.

PATIENTS AND METHODS

ELISA was applied to quantify concentrations of AMP RNase-7, psoriasin, hBD-2, hBD-3 and LL-37 in nasal secretions of 20 healthy volunteers. Immunohistochemistry was used to detect the local cellular sources of AMP in the vestibulum nasi (squamous epithelium) and compared to the mucosa of the turbinates (pseudostratified epithelium) in 10 healthy volunteers.

RESULTS

Expression of RNase 7 and psoriasin was detected in all nasal secretion specimens, whereas LL-37 was detected in 16, hBD-2 in 5 and hBD-3 in 6 specimens. In the vestibulum nasi, luminal cell layers were demonstrated as local cellular sources for hBD-3 and RNase 7, whereas psoriasin was found in all layers of the stratified squamous epithelium. LL-37 was detected in 1 stroma cells sample, whereas hBD-2 was not detected at all. In turbinate biopsie,s hBD-3 and LL-37 were detectable in the epithelium, stroma cells and submucosal glands. RNase 7 was only present in submucosal glands. HBD-2 and psoriasin were not detected.

CONCLUSION

These data demonstrate that the nasal epithelium contains a chemical defense shield through the expression and secretion of various AMP.

摘要

目的

完整的鼻黏膜屏障是上呼吸道系统发挥功能防御的前提，特别是对吸入的潜在有害微生物的持续威胁。抗菌肽（AMP）在维持屏障功能方面起着重要作用。关于鼻腔黏膜的 AMP 数据很少。本研究旨在进一步深入了解根据前庭和鼻甲的定义解剖区域，AMP 的差异表达和分泌模式。

患者和方法

ELISA 用于定量 20 名健康志愿者的鼻分泌物中 AMP RNase-7、角鲨烯、hBD-2、hBD-3 和 LL-37 的浓度。免疫组织化学用于检测前庭（鳞状上皮）和鼻甲（假复层上皮）中 AMP 的局部细胞来源，并与 10 名健康志愿者进行比较。

结果

在所有鼻分泌物标本中均检测到 RNase 7 和角鲨烯的表达，而 LL-37 在 16 个标本中、hBD-2 在 5 个标本中、hBD-3 在 6 个标本中检测到。在前庭中，腔细胞层被证明是 hBD-3 和 RNase 7 的局部细胞来源，而角鲨烯存在于分层鳞状上皮的所有层中。LL-37 在 1 个基质细胞样本中检测到，而 hBD-2 则根本未检测到。在鼻甲活检中，hBD-3 和 LL-37 可在上皮、基质细胞和粘膜下腺中检测到。RNase 7 仅存在于粘膜下腺中。未检测到 hBD-2 和角鲨烯。