子痫前期合并胎盘组织中细胞凋亡及Bcl-2、Bax、p53、caspase-3、Fas、Fas配体的表达
Apoptosis and expression of Bcl-2, Bax, p53, caspase-3, and Fas, Fas ligand in placentas complicated by preeclampsia.
作者信息
Mendilcioglu I, Karaveli S, Erdogan G, Simsek M, Taskin O, Ozekinci M
机构信息
Obstetrics and Gynecology Department, Akdeniz University, Antalya, Turkey.
出版信息
Clin Exp Obstet Gynecol. 2011;38(1):38-42.
AIM
To determine whether preeclampsia is associated with an increase in placental apoptosis and altered expression of mediators of apoptosis such as Fas, Fas ligand, caspase-3, p53, Bcl-2 and Bax.
METHODS
Placental samples obtained from 20 women with preeclampsia and 14 normal women were analyzed using the Klenow, Frag-EL DNA fragmentation detection kit for apoptosis. Expression of Fas, Fas ligand, caspase-3, p53 Bcl-2, and Bax was assessed using immunohistochemistry.
RESULTS
The median percentage of apoptotic nuclei in trophoblasts was significantly higher for the preeclampsia group than for the controls (6.9 vs 0.25; p =.001). Fas ligand expression was significantly higher in the decidua among the subjects with preeclampsia compared with controls (1.2 vs 0; p = .023). Otherwise no difference was observed in the expression of Bax , Bcl-2, p53, caspase-3, and Fas between groups.
CONCLUSION
Increased apoptosis in preeclampsia may not be associated with significant alterations in Fas, Fas ligand, caspase-3, p53, Bcl-2 and Bax expression.
目的
确定子痫前期是否与胎盘细胞凋亡增加以及凋亡介质如Fas、Fas配体、半胱天冬酶-3、p53、Bcl-2和Bax的表达改变有关。
方法
使用Klenow、Frag-EL DNA片段化检测试剂盒对从20例子痫前期妇女和14例正常妇女获取的胎盘样本进行细胞凋亡分析。采用免疫组织化学法评估Fas、Fas配体、半胱天冬酶-3、p53、Bcl-2和Bax的表达。
结果
子痫前期组滋养层细胞中凋亡细胞核的中位数百分比显著高于对照组（6.9对0.25；p = 0.001）。与对照组相比，子痫前期患者蜕膜中Fas配体的表达显著更高（1.2对0；p = 0.023）。此外，两组之间Bax、Bcl-2、p53、半胱天冬酶-3和Fas的表达未观察到差异。
结论
子痫前期细胞凋亡增加可能与Fas、Fas配体、半胱天冬酶-3、p53、Bcl-2和Bax表达的显著改变无关。
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