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[人网状细胞培养中异质质体的形成]

[Formation of heterosymplasts in human reticular cell cultures].

作者信息

Khesin Ia E, Voronina F V, Amchenkova A M, Gulevich N E, Sovetova G P

出版信息

Tsitologiia. 1978 Sep;20(9):1065-9.

PMID:214917
Abstract

The cell fusion has been studied in human reticular cell cultures J-96 and J-41 treated with the Sendai virus or with polyethylene glycol 1000 and 6000. The J-96 cells have a high alkaline phosphatase activity, in J-41 cells the enzyme is not detectable. No heterogenous alkaline phosphatase activity was seen in the protoplasm of symplasts 18 hours after virus cell fusion. It has been shown with polyethylene glycol treatment that during the fusion of cells J-96 and J-41 the enzyme activity was spreading over the symplast protoplasm.

摘要

在人网状细胞培养物J - 96和J - 41中,研究了经仙台病毒或聚乙二醇1000和6000处理后的细胞融合情况。J - 96细胞具有高碱性磷酸酶活性,而在J - 41细胞中未检测到该酶。病毒介导的细胞融合18小时后,在合胞体原生质中未观察到异质性碱性磷酸酶活性。聚乙二醇处理表明,在J - 96和J - 41细胞融合过程中,酶活性扩散到合胞体原生质中。

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