Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, USA.
Curr Opin Chem Biol. 2011 Jun;15(3):452-7. doi: 10.1016/j.cbpa.2011.03.014. Epub 2011 Apr 12.
Computational and theoretical methods are advancing protein design as a means to create and investigate proteins. Such efforts further our capacity to control, design and understand biomolecular structure, sequence and function. Herein, the focus is on some recent applications that involve using theoretical and computational methods to guide the design of protein sequence ensembles, new enzymes, proteins with novel cofactors, and membrane proteins.
计算和理论方法正在推动蛋白质设计的发展,作为创造和研究蛋白质的一种手段。这些努力进一步提高了我们控制、设计和理解生物分子结构、序列和功能的能力。本文重点介绍了一些最近的应用,这些应用涉及使用理论和计算方法来指导蛋白质序列集合、新酶、具有新型辅因子的蛋白质和膜蛋白的设计。