肥胖妇女的胎儿血清叶酸浓度与胎盘叶酸转运。
Fetal serum folate concentrations and placental folate transport in obese women.
机构信息
Division of Maternal Fetal Medicine, University of Texas Health Science Center at San Antonio, Department of Obstetrics & Gynecology, San Antonio, TX 78229, USA.
出版信息
Am J Obstet Gynecol. 2011 Jul;205(1):83.e17-25. doi: 10.1016/j.ajog.2011.02.053. Epub 2011 Feb 23.
OBJECTIVE
We hypothesized that fetal folate serum concentrations are lower and placental folate transport is impaired in pregnancies of obese women.
STUDY DESIGN
Umbilical vein serum and placental tissue were collected from normal weight and obese pregnant women at term. Cellular localization (immunohistochemistry) of folate receptor-α (FR-α), proton coupled folate transporter (PCFT), and reduced folate carrier (RFC) was established. Protein expression (Western blot) and transporter activity (isotope labeled methyltetrahydrofolate) were determined in syncytiotrophoblast microvillus membranes (MVM).
RESULTS
Fetal folate concentrations were similar in obese women as compared with normal weight women. Protein expression of FR-α in microvillus membranes was increased (+173%), in RFC was decreased (-41%), and in PCFT was unchanged. However, activity of FR-α, PCFT, and RFC was unaltered in obesity.
CONCLUSION
Fetal serum folate concentrations and placental folate transport activity are not altered in obesity at term, which suggests that limited availability of folate does not contribute to abnormal gene methylation and developmental programming.
目的
我们假设肥胖孕妇的胎儿血清叶酸浓度较低，胎盘叶酸转运受损。
研究设计
足月时从正常体重和肥胖孕妇的脐静脉血清和胎盘组织中采集样本。建立叶酸受体-α（FR-α）、质子偶联叶酸转运体（PCFT）和还原叶酸载体（RFC）的细胞定位（免疫组织化学）。在合体滋养细胞微绒毛膜（MVM）中测定蛋白表达（Western blot）和转运体活性（同位素标记的亚甲基四氢叶酸）。
结果
与正常体重妇女相比，肥胖妇女的胎儿叶酸浓度相似。微绒毛膜中 FR-α 的蛋白表达增加（增加 173%），RFC 减少（减少 41%），而 PCFT 不变。然而，肥胖并没有改变 FR-α、PCFT 和 RFC 的活性。
结论
足月肥胖并不改变胎儿血清叶酸浓度和胎盘叶酸转运活性，这表明有限的叶酸供应不会导致异常的基因甲基化和发育编程。
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