薏苡（Coix lachryma-jobi L. var. ma-yuen Stapf）对胃癌 AGS 细胞系生长和消炎痛诱导的胃溃疡的胃保护活性。

Gastroprotective activities of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) on the growth of the stomach cancer AGS cell line and indomethacin-induced gastric ulcers.

机构信息

Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan.

出版信息

J Agric Food Chem. 2011 Jun 8;59(11):6025-33. doi: 10.1021/jf2009556. Epub 2011 May 5.

DOI:10.1021/jf2009556

Abstract

Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) seeds have long been used to treat warts, chapped skin, rheumatism, and neuralgia in traditional Chinese medicine (TCM). Recently, studies demonstrated its anti-inflammatory, antiproliferative, antitumor, and antiallergic activities. In the present study, we first report the gastroprotective effects of dehulled adlay (DA) seeds, which consist of bran (AB) and endosperm (AE). The DA ethanolic extract (DAE) was prepared, along with the AB and AE ethanolic extracts (ABE and AEE), and the inhibitory effects of these extracts were tested on the AGS gastric cancer cell line. Results indicated that the ABE showed better antiproliferative activity, and 19 compounds were purified from AB in a further phenolic-compound-guided separation. Among the isolated compounds, caffeic and chlorogenic acids significantly suppressed the growth of AGS cells. In addition, the antiulcer activity of DA was examined in an indomethacin-induced gastric lesion model. The ulcer index (UI) and oxidative biomarkers in animals decreased, while the non-protein sulfhydryl (NPSH) groups were elevated when given DA. This is the first investigation of antiulcer activity of adlay, and we demonstrated that the antioxidative-active phenolic acids in DA contribute to some portion of the gastroprotective effects.

摘要

薏苡（Coix lachryma-jobi L. var. ma-yuen Stapf）种子长期以来一直被用于传统中药（TCM）治疗疣、皲裂皮肤、风湿和神经痛。最近的研究表明，它具有抗炎、抗增殖、抗肿瘤和抗过敏活性。在本研究中，我们首次报道了去壳薏苡（DA）种子的胃保护作用，其包括糠（AB）和胚乳（AE）。制备了 DA 乙醇提取物（DAE），以及 AB 和 AE 乙醇提取物（ABE 和 AEE），并测试了这些提取物对 AGS 胃癌细胞系的抑制作用。结果表明，ABE 显示出更好的抗增殖活性，并且在进一步的酚类化合物导向分离中从 AB 中分离出 19 种化合物。在分离出的化合物中，咖啡酸和绿原酸显著抑制了 AGS 细胞的生长。此外，还在消炎痛诱导的胃损伤模型中研究了 DA 的抗溃疡活性。当给予 DA 时，动物的溃疡指数（UI）和氧化生物标志物降低，而非蛋白巯基（NPSH）组升高。这是对薏苡抗溃疡活性的首次研究，我们证明了 DA 中具有抗氧化活性的酚酸部分有助于胃保护作用。