体外检测嗜内孢霉属对大环内酯类和四环素类抗生素的敏感性。
In vitro susceptibility of Pythium insidiosum to macrolides and tetracycline antibiotics.
机构信息
Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
出版信息
Antimicrob Agents Chemother. 2011 Jul;55(7):3588-90. doi: 10.1128/AAC.01586-10. Epub 2011 May 2.
Abstract
We describe the in vitro activity of macrolides and tetracycline antibiotics against Pythium insidiosum. The MICs were determined according to CLSI procedures (visual MIC) and by a colorimetric method [3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT)]. The lowest geometric mean (GM) MIC (MICs in μg/ml) (0.39 and 0.7 by visual reading and colorimetric method, respectively) and MIC ranges (0.125 to 2.0) were obtained for minocycline, while the highest MICs were shown for erythromycin (GM of 7.58 and 12.25 by visual reading and colorimetric method, respectively, and MIC ranged from 2 to 32). This significant in vitro activity makes these classes of antibiotics good candidates for experimental treatment of pythiosis.
摘要
我们描述了大环内酯类和四环素类抗生素对暗孢节旋菌的体外活性。根据 CLSI 程序（目测 MIC）和比色法 [3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴化盐（MTT）] 来确定 MIC。米诺环素的最低几何均数（GM）MIC（以 μg/ml 表示）（分别通过目测和比色法为 0.39 和 0.7）和 MIC 范围（0.125 至 2.0）最小，而红霉素的 MIC 最高（分别通过目测和比色法为 7.58 和 12.25，MIC 范围为 2 至 32）。这种显著的体外活性使这些抗生素类别成为治疗潜隐体病的实验治疗的良好候选药物。
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