Centro Hispano-Luso de Investigaciones Agrarias (C.IALE.), Universidad de Salamanca, Villamayor (Salamanca), Spain.
J Investig Allergol Clin Immunol. 2011;21(3):222-8.
Patients with pollinosis show allergic symptoms related to airborne pollen levels, although this association is not always close. The use of new diagnostic techniques could improve our knowledge of this relationship.
To evaluate the relationship between pollen counts and the results obtained using 2 diagnostic techniques: the skin prick test and allergen-specific immunoglobulin E (slgE) concentrations in serum.
Sixty-eight pollen-allergic patients were diagnosed using a combination of the high-capacity screening approach ADVIA Centaur with a panel of 13 purified allergens and a skin prick test (SPT) with conventional extracts. Pollen levels were obtained by means of a volumetric sampler.
The highest percentages of sensitization were detected for grass mixture allergens and major recombinant grass allergens (Phl p 1 and Phi p 5), followed by olive tree extracts and olive allergens (Ole e 1 and Ole e 9), in SPT and using recombinant allergens, respectively. The main pollen types registered in the atmosphere during 2006 and 2007 were Quercus, Poaceae, and Cupressaceae. A statistically significant correlation was observed between total pollen levels and median values of slgE, especially in 2007.
A strong and significant positive correlation was found between pollen counts and slgE levels. This correlation was weaker in the case of SPT and airborne pollen.
花粉症患者表现出与空气中花粉水平相关的过敏症状，尽管这种关联并不总是密切的。使用新的诊断技术可以提高我们对这种关系的认识。
评估花粉计数与两种诊断技术结果之间的关系：皮肤点刺试验和血清过敏原特异性免疫球蛋白 E（slgE）浓度。
使用高容量筛选方法 ADVIA Centaur 与 13 种纯化过敏原组合以及常规提取物进行皮肤点刺试验（SPT），对 68 例花粉过敏患者进行诊断。通过体积采样器获得花粉水平。
在 SPT 和使用重组过敏原时，草混合物过敏原和主要重组草过敏原（Phl p 1 和 Phi p 5）的致敏率最高，其次是橄榄树提取物和橄榄过敏原（Ole e 1 和 Ole e 9）。2006 年和 2007 年空气中登记的主要花粉类型为栎属、禾本科和柏科。总花粉水平与 slgE 的中位数之间存在统计学上显著的相关性，尤其是在 2007 年。
发现花粉计数与 slgE 水平之间存在强烈而显著的正相关。在 SPT 和空气中的花粉情况下，这种相关性较弱。
