利用新型单克隆抗体CCH2在细胞培养中早期检测巨细胞病毒

Early detection of cytomegalovirus in cell culture by a new monoclonal antibody, CCH2.

作者信息

Zweygberg Wirgart B, Landqvist M, Hökeberg I, Eriksson B M, Olding-Stenkvist E, Grillner L

机构信息

Department of Clinical Microbiology, Section of Virology, Karolinska Hospital, Stockholm, Sweden.

出版信息

J Virol Methods. 1990 Feb;27(2):211-9. doi: 10.1016/0166-0934(90)90137-5.

DOI:10.1016/0166-0934(90)90137-5

PMID:2156881

Abstract

A CMV monoclonal antibody, CCH2, produced in this laboratory was evaluated for rapid detection of CMV. Two staining procedures, immunofluorescence and an immunoenzymatic technique using biotin-streptavidin peroxidase, were compared. The CCH2 monoclonal antibody was used to demonstrate early CMV antigen in cell culture 24 h after inoculation of 598 urine samples from kidney transplanted patients by indirect immunofluorescence in comparison with virus isolation. One hundred and sixty of the specimens were stained additionally by an immunoenzymatic technique and the results were compared. CMV was isolated from 170 out of 598 specimens within 6 weeks. Early CMV antigen was demonstrated in 114 of these specimens by immunofluorescence giving a sensitivity of 67% and a specificity of 95%. In the comparison with the immunoenzymatic staining procedure the results for all three tests agreed for 81% (130/160) of the specimens. After resolving discordant results into true positives and true negatives, the sensitivity was 87, 85 and 70%, respectively for virus isolation, immunoenzymatic staining and immunofluorescence and the specificity 100, 96 and 99%. The CCH2 monoclonal antibody proved to be useful for rapid detection of CMV in urine specimens and using immunoenzymatic staining with biotin-streptavidin a sensitivity comparable to that of virus isolation was found.

摘要

对本实验室制备的巨细胞病毒（CMV）单克隆抗体CCH2进行了检测CMV的快速评估。比较了两种染色方法，即免疫荧光法和使用生物素 - 链霉亲和素过氧化物酶的免疫酶技术。通过间接免疫荧光法，使用CCH2单克隆抗体检测598例肾移植患者尿液样本接种后24小时细胞培养中的早期CMV抗原，并与病毒分离法进行比较。另外160份标本采用免疫酶技术染色并比较结果。在6周内，598份标本中有170份分离出CMV。通过免疫荧光法在其中114份标本中检测到早期CMV抗原，灵敏度为67%，特异性为95%。与免疫酶染色方法比较，所有三项检测结果在81%（130/160）的标本中一致。在将不一致的结果解析为真阳性和真阴性后，病毒分离、免疫酶染色和免疫荧光的灵敏度分别为87%、85%和70%，特异性分别为100%、96%和99%。结果证明，CCH2单克隆抗体可用于尿液标本中CMV的快速检测，并且使用生物素 - 链霉亲和素免疫酶染色法可获得与病毒分离法相当的灵敏度。