何首乌水培繁殖及不定根形成过程中生理生化特性的动态变化

[Water cultured propagation of Polygonum multiflorum and dynamic changes of physiological and biochemical characteristics during adventitious roots formation].

作者信息

Gao Jie, Liu Xiaohui, Yuan Yike

机构信息

College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Feb;36(3):375-8.

PMID:21585047

Abstract

Water cultured propagation technology of Polygonum multiflorum was investigated with Rooting Powder No. 2 (ABT 2) comparison experiments, and the dynamic changes of endogenous hormones including indole acetic acid (IAA), abscisic acid (ABA), zeatin riboside (ZRs) contents and activities of indoleacetic acid oxidase (IAAO), polyphenol oxidase (PPO) were analyzed during rooting period. The results showed that rooting percentage of softwood cutting with 50 mg x L(-1) ABT2 and 10 mg x L(-1) ABT2 + 0.2% Urea + 0.2% KH2PO4 treatments was 94%, rooting percentage of softwood cuttings of control was 46% only. The adventitious rooting displayed three distinct phases i. e. root-inducing, root formation and root-elongating phases. The dynamic changes of contents of endogenous plant hormones (IAA, ABA, ZRs) and activities of IAAO, PPO tested were tightly related to the rooting process of soft-wood cuttings in P. multiflorum. During root-inducing phase the contents of IAA, ABA and ZRs decreased sharply, whereas ZRs content and IAAO activity kept higher level. IAA content reached the peak and PPO activity increased obviously during root formation phase, while IAAO activities and ABA, ZRs contents declined to minimum. During root-elongating phase PPO, IAAO activities were higher and IAA, ABA, ZRs contents kept steady. During rooting ABT2 (50 mg x L(-1)) treatment increased the content of IAA and PPO activity in cuttings, while the opposite result occurred in contents of ZRs, ABA and IAAO activity.

摘要

以2号生根粉（ABT 2）进行对比试验，研究何首乌水培繁殖技术，并分析生根期内吲哚乙酸（IAA）、脱落酸（ABA）、玉米素核苷（ZRs）等内源激素含量以及吲哚乙酸氧化酶（IAAO）、多酚氧化酶（PPO）活性的动态变化。结果表明，50 mg·L⁻¹ ABT2处理和10 mg·L⁻¹ ABT2 + 0.2%尿素 + 0.2%磷酸二氢钾处理的嫩枝扦插生根率为94%，而对照嫩枝扦插生根率仅为46%。不定根形成表现为明显的3个阶段，即诱导期、形成期和伸长期。所测内源植物激素（IAA、ABA、ZRs）含量及IAAO、PPO活性的动态变化与何首乌嫩枝扦插生根过程密切相关。在诱导期，IAA、ABA和ZRs含量急剧下降，而ZRs含量和IAAO活性保持较高水平。在根形成期，IAA含量达到峰值，PPO活性明显增加，而IAAO活性以及ABA、ZRs含量降至最低。在根伸长期，PPO、IAAO活性较高，IAA、ABA、ZRs含量保持稳定。生根过程中，ABT2（50 mg·L⁻¹）处理提高了插穗中IAA含量和PPO活性，而ZRs、ABA含量及IAAO活性则呈现相反的变化结果。