DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Via Fratelli Rosselli 107, 42100 Reggio Emilia, Italy Centro di Ecologia Alpina, Viote del Monte Bondone, 38040 Trento, Italy Department of Botany and Zoology, Evolutionary Genomics Group, University of Stellenbosch, PB X1, Matieland 7602, South Africa.
Mol Ecol Resour. 2008 Nov;8(6):1294-6. doi: 10.1111/j.1755-0998.2008.02167.x. Epub 2008 Sep 15.
We describe a new method for the sex determination of tissue originating from Oryctolagus cuniculus (European rabbit), Lepus europaeus (European brown hare) and Lepus timidus (mountain hare) based on PCR-RFLP analysis of point mutations that differentiate the ZFX and ZFY gene sequences. Among several applications, this PCR-RFLP method could be used to investigate gender ratio and evaluate the population dynamics of these species using samples collected when sex cannot be identified.
我们描述了一种新的方法，用于鉴定来自欧洲兔（Oryctolagus cuniculus）、欧洲野兔（Lepus europaeus）和山地野兔（Lepus timidus）的组织的性别，该方法基于 ZFX 和 ZFY 基因序列中区分点突变的 PCR-RFLP 分析。在多种应用中，这种 PCR-RFLP 方法可用于研究性别比例，并使用无法识别性别的样本评估这些物种的种群动态。
