慢性刺激诱导的效应表明，碳酸酐酶III和慢肌球蛋白重链在骨骼肌中存在协同表达。

Chronic stimulation-induced effects point to a coordinated expression of carbonic anhydrase III and slow myosin heavy chain in skeletal muscle.

作者信息

Jeffery S, Kelly C D, Carter N, Kaufmann M, Termin A, Pette D

机构信息

Department of Child Health, St. George's Hospital Medical School, London, UK.

出版信息

FEBS Lett. 1990 Mar 26;262(2):225-7. doi: 10.1016/0014-5793(90)80195-o.

DOI:10.1016/0014-5793(90)80195-o

PMID:2159412

Abstract

Chronic low-frequency stimulation of rat fast-twitch muscle induces 3.7-fold elevations in cytochrome c oxidase activity, but remains without effect on carbonic anhydrase III (CAIII) mRNA and protein. This is in contrast with the situation in the rabbit where chronic stimulation elicits more than 10-fold elevations in CAIII activity and mRNA content which coincide with an enhanced expression of the slow myosin heavy chain (HCI). Since chronic stimulation of rat muscle does not enhance the expression of HCI, we conclude that CAIII is expressed in parallel with HCI and, therefore, is present only in type I and C fibers.

摘要

对大鼠快肌进行慢性低频刺激可使细胞色素c氧化酶活性升高3.7倍，但对碳酸酐酶III（CAIII）的mRNA和蛋白质无影响。这与兔子的情况形成对比，在兔子中，慢性刺激可使CAIII活性和mRNA含量升高10倍以上，这与慢肌球蛋白重链（HCI）表达增强相一致。由于对大鼠肌肉的慢性刺激不会增强HCI的表达，我们得出结论，CAIII与HCI平行表达，因此仅存在于I型和C型纤维中。