IVF 中的生物 pH 缓冲剂：对成功是帮助还是阻碍。
Biological pH buffers in IVF: help or hindrance to success.
机构信息
Department of Obstetrics & Gynecology, University of Michigan, Ann Arbor, MI 48108, USA.
出版信息
J Assist Reprod Genet. 2011 Aug;28(8):711-24. doi: 10.1007/s10815-011-9582-0. Epub 2011 May 26.
PURPOSE
Minimizing environmental stress helps maintain cellular homeostasis and is a crucial component in optimizing embryo development in vitro and resulting ART success. One stressor of particular interest is pH. Biologic buffers, such as HEPES and MOPS, are valuable tools for stabilizing pH. The objective of this manuscript is to summarize efficacy and impact of various pH buffers used during IVF lab procedures
METHODS
Keyword searches were performed using Pubmed and Medline and relevant literature reviewed.
RESULTS
Various pH buffers have been used with varying degrees of success for gamete and embryo processing in a variety of animal species, as well as in human.
CONCLUSION
Though biologic buffers off a means to improve pH stability, not all buffers may be appropriate for use with gametes and embryos. Specific buffers may have undesired effects, and these may be buffer, species, cell type or concentration dependent. Continued research is needed to further refine and improve the use of biologic buffers for use in human ART.
目的
减轻环境压力有助于维持细胞内稳态，是优化体外胚胎发育和提高辅助生殖技术（ART）成功率的关键因素。其中一个特别引人关注的应激源是 pH 值。生物缓冲剂，如 HEPES 和 MOPS，是稳定 pH 值的有价值工具。本文的目的是总结在体外受精实验室程序中使用各种 pH 缓冲剂的效果和影响。
方法
使用 Pubmed 和 Medline 进行关键词搜索，并回顾相关文献。
结果
在各种动物物种以及人类中，已经使用了各种 pH 缓冲剂来处理配子和胚胎，但效果不同。
结论
虽然生物缓冲剂提供了改善 pH 值稳定性的方法，但并非所有缓冲剂都适用于配子和胚胎。特定的缓冲剂可能会产生不良影响，这些影响可能取决于缓冲剂、物种、细胞类型或浓度。需要进一步的研究来进一步改进和优化生物缓冲剂在人类 ART 中的应用。
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