[汉族人群血清淀粉样蛋白A1基因多态性与颈动脉内膜中层厚度的相关性]

[Association between serum amyloid protein A1 polymorphisms and carotid intima media thickness in Han Chinese].

作者信息

Xie Xiang, Ma Yi-tong, Yang Yi-ning, Fu Zhen-yan, Li Xiao-mei, Ma Xiang, Huang Ding, Liu Fen, Chen Bang-dang, Xiang Yang, Huang Ying

机构信息

Heart Center, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China.

出版信息

Zhonghua Xin Xue Guan Bing Za Zhi. 2011 Apr;39(4):320-4. doi: 10.3760/cma.j.issn.0253-3758.2011.04.008.

DOI:10.3760/cma.j.issn.0253-3758.2011.04.008

PMID:21624307

Abstract

OBJECTIVE

To explore the association between genetic polymorphism of serum amyloid protein A1 (SAA1) with carotid intima media thickness in a healthy Han Chinese population of Xinjiang.

METHODS

A total of 449 healthy Han Chinese participating the cardiovascular risk survey between June 2007 and September 2009 were included, the genotypes of the SAA1 were detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The mean IMT of the right and left common carotid arteries were measured by B-mode ultrasonography.

RESULTS

(1) There was strong linkage disequilibrium between rs12218 and rs2229338 (D' = 0.89). (2) The carotid common IMT (CC-IMT) and the carotid bulb IMT (CB-IMT) were similar between the AA genotype (wild genotype) and the GGFAG genotype (mutational genotype) in rs2229338 of SAA1 gene. (3) CC-IMT [(0.081 ± 0.071) cm vs (0.068 ± 0.019) cm, P = 0.01] was significantly thicker in CC + CT genotype (mutational genotype) group than in TT genotype (wild genotype) of rs12218 group and the difference remains significant after adjustment for age, gender, blood pressure, waist circumference, creatinine and high density lipoprotein cholesterol. CB-IMT [(0.085 ± 0.038) cm vs. (0.081 ± 0.052) cm, P = 0.36] was similar between CC + CT genotype and TT genotype of rs12218 groups.

CONCLUSION

Our results suggested that the genetic polymorphism of SAA1 might be linked with IMT and rs12218 mutation could serve as a promoting factor for IMT in Han Chinese people.

摘要

目的

探讨新疆健康汉族人群血清淀粉样蛋白A1（SAA1）基因多态性与颈动脉内膜中层厚度之间的关联。

方法

纳入2007年6月至2009年9月期间参与心血管风险调查的449名健康汉族人，采用聚合酶链反应和限制性片段长度多态性（PCR-RFLP）检测SAA1的基因型。用B型超声测量左右颈总动脉的平均内膜中层厚度（IMT）。

结果

（1）rs12218与rs2229338之间存在强连锁不平衡（D' = 0.89）。（2）SAA1基因rs2229338位点的AA基因型（野生基因型）和GGFAG基因型（突变基因型）之间的颈总动脉IMT（CC-IMT）和颈动脉球部IMT（CB-IMT）相似。（3）rs12218位点的CC + CT基因型（突变基因型）组的CC-IMT[（0.081±0.071）cm比（0.068±0.019）cm，P = 0.01]显著厚于TT基因型（野生基因型）组，在调整年龄、性别、血压、腰围、肌酐和高密度脂蛋白胆固醇后差异仍显著。rs12218位点的CC + CT基因型和TT基因型组之间的CB-IMT[（0.085±0.038）cm比（0.081±0.052）cm，P = 0.36]相似。