利用敲低方法鉴定结核分枝杆菌中的脆弱途径。
Identifying vulnerable pathways in Mycobacterium tuberculosis by using a knockdown approach.
机构信息
Queen Mary University of London, Barts and the London, London E1 2AT, United Kingdom.
出版信息
Appl Environ Microbiol. 2011 Jul;77(14):5040-3. doi: 10.1128/AEM.02880-10. Epub 2011 Jun 3.
Abstract
We constructed recombinant strains of Mycobacterium tuberculosis in which expression of specific genes was downregulated to identify vulnerable drug targets. Growth phenotypes in macrophages and culture were used to rank targets: the dprE1, clpP1, and fadD32 operons were the best targets and glnA1, glnE, pknL, regX3, and senX3 were poor targets.
摘要
我们构建了结核分枝杆菌的重组菌株，下调特定基因的表达以鉴定易受药物影响的靶点。利用巨噬细胞和培养物中的生长表型对靶点进行排名：dprE1、clpP1 和 fadD32 操纵子是最佳靶点，glnA1、glnE、pknL、regX3 和 senX3 是较差的靶点。
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