Department of Agricultural Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, Taiwan.
J Chromatogr A. 2011 Jul 22;1218(29):4709-13. doi: 10.1016/j.chroma.2011.05.056. Epub 2011 May 23.
An improved normal phase high performance liquid chromatographic (NP-HPLC) method was developed for simultaneous quantification of eight vitamin E isomers (α-, β-, γ- and δ-tocopherols and α-, β-, γ- and δ-tocotrienols) and γ-oryzanol in rice. A complete separation of all compounds was achieved within 25 min using an Inertsil CN-3, SIL-100A 5 μM (4.6 mm × 250 mm) column and an isocratic elution system of hexane/isopropanol/ethylacetate/acetic acid (97.6:0.8:0.8:0.8, v/v/v/v) at a flow rate varying from 0.7 to 1.5 mL min(-1). A linear correlation coefficient (r(2)>0.99) and high reproducibility were obtained at concentrations ranging 0.05-10 μg mL(-1) for vitamin E isomers and 0.5-500 μg mL(-1) for γ-oryzanol. This method proved to be rapid, accurate and reproducible.
建立了一种改进的正相高效液相色谱法（NP-HPLC），用于同时定量分析大米中的 8 种维生素 E 异构体（α-、β-、γ-和δ-生育酚和α-、β-、γ-和δ-生育三烯醇）和γ-谷维素。使用 Inertsil CN-3、SIL-100A 5 μM（4.6 mm×250 mm）柱和正相洗脱系统（己烷/异丙醇/乙酸乙酯/乙酸（97.6:0.8:0.8:0.8，v/v/v/v）），在 0.7 至 1.5 mL min(-1) 的流速下，可在 25 min 内实现所有化合物的完全分离。在 0.05-10 μg mL(-1) 范围内，维生素 E 异构体的线性相关系数（r(2)>0.99）和重现性均较高，而在 0.5-500 μg mL(-1) 范围内，γ-谷维素的重现性均较高。该方法快速、准确且重现性好。
