Traub P, Traub U
Mol Biol Rep. 1978 Oct 16;4(3):131-5. doi: 10.1007/BF00777512.
The effect of mengovirus infection on the extent of phosphorylation of histone H1 was studied in Ehrlich ascites tumor cells. After prelabeling of the nuclear protein with [32P] orthophosphate, the excorporation of radioactivity was followed as a function of time postinfection. Employing high-resolution polyacrylamide gradient slab gel electrophoresis and autoradiography, it was found that, compared to a relatively slow turnover of phosphate groups in histone H1 in mock-infected cells, in mengovirus-infected cells the excorporation of radiolabel from histone H1 was significantly enhanced. In the latter case, the decrease of histone-bound radioactivity was paralleled by a reduction of the band multiplicity in the histone H1 region of the electrophoresis profile. It was also shown that the microheterogeneity in the histone H1 complements isolated at various times postinfection was reduced to the same basal 3-band level by incubation of the nuclear protein fractions in the presence of alkaline phosphatase. After this treatment, the band multiplicity equaled that found in histone H1 from stationary cells.
在艾氏腹水瘤细胞中研究了脑心肌炎病毒感染对组蛋白H1磷酸化程度的影响。在用[32P]正磷酸盐对核蛋白进行预标记后，跟踪放射性物质的外排随感染后时间的变化。采用高分辨率聚丙烯酰胺梯度平板凝胶电泳和放射自显影法，发现与 mock 感染细胞中组蛋白H1中磷酸基团相对缓慢的周转相比，在脑心肌炎病毒感染的细胞中，组蛋白H1中放射性标记的外排显著增强。在后一种情况下，组蛋白结合放射性的降低与电泳图谱中组蛋白H1区域条带多样性的减少平行。还表明，通过在碱性磷酸酶存在下孵育核蛋白组分，在感染后不同时间分离的组蛋白H1补体中的微异质性降低到相同的基础3条带水平。经过这种处理后，条带多样性与静止细胞中组蛋白H1中的条带多样性相等。
