King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
Int J Gynecol Cancer. 2011 Jul;21(5):907-10. doi: 10.1097/IGC.0b013e318214219f.
To determine the rates and types of human papilloma virus (HPV) infection in cervical cancer specimens from Saudi patients.
One hundred specimens were randomly selected and retrieved from the achieved samples stored in the pathology department accessioned under the diagnosis of cervical cancer and carcinoma in situ between the years 1997 and 2007. Human papilloma virus in the clinical samples was detected using polymerase chain reaction amplification methods. Two primer systems are commonly used: the MY09-MY11 primers and the GP5+-GP6+ that amplify a wide range of HPV genotypes. Human papilloma virus isolates were genotyped using DNA sequencing and reverse line blot hybridization assay to identify the high-risk HPV genotypes.
Ninety cases fulfilled the diagnostic criteria and were analyzed. The rate of HPV genotype detection among cervical cancer samples was 95.5%. The most common HPV genotype detected by both methods was HPV-16 (63.4%), followed by HPV-18 (11.1%), HPV-45 (4.5%), HPV-33 (3.3%), and HPV-31, HPV-52, HPV-53, HPV-58, HPV-59, and HPV-66 with 2.2% prevalence rate each.
Prevalence of HPV genotypes among patients with cervical cancer in Saudi Arabia is comparable to the international rates. The use of the reverse line blot hybridization assay genotyping method could be useful for classifying oncogenic HPV-positive women. It is relatively inexpensive and reliable and can be performed in routine practice or epidemiological study compared with the available standard commercial kits.
确定沙特患者宫颈癌标本中人乳头瘤病毒（HPV）感染的发生率和类型。
从 1997 年至 2007 年间病理科保存的已诊断为宫颈癌和原位癌的病例中随机选择并提取 100 例标本。采用聚合酶链反应扩增法检测临床样本中的人乳头瘤病毒。两种引物系统通常用于检测 HPV：MY09-MY11 引物和 GP5+-GP6+，它们可扩增广泛的 HPV 基因型。使用 DNA 测序和反向线杂交分析对 HPV 分离株进行基因分型，以鉴定高危 HPV 基因型。
符合诊断标准的 90 例进行了分析。宫颈癌样本中 HPV 基因型的检出率为 95.5%。两种方法均检测到的最常见 HPV 基因型是 HPV-16（63.4%），其次是 HPV-18（11.1%）、HPV-45（4.5%）、HPV-33（3.3%）和 HPV-31、HPV-52、HPV-53、HPV-58、HPV-59 和 HPV-66，其流行率分别为 2.2%。
在沙特阿拉伯的宫颈癌患者中 HPV 基因型的流行率与国际水平相当。反向线杂交分析基因分型方法的应用对于分类致癌 HPV 阳性女性可能是有用的。与现有的标准商业试剂盒相比，它相对便宜、可靠，并且可以在常规实践或流行病学研究中进行。
