α-和β-肾上腺素能阻滞剂对促甲状腺素与脂肪细胞膜结合的影响。

Effects of alpha- and beta-adrenergic blockers on binding of thyrotrophin to fat cell membrane.

作者信息

Kishihara M, Nakao Y, Baba Y, Ohgo S, Matsukura S, Fujita T, Kuma K

出版信息

Acta Endocrinol (Copenh). 1979 Feb;90(2):240-8. doi: 10.1530/acta.0.0900240.

DOI:10.1530/acta.0.0900240

Abstract

Alpha- (phentolamine) and beta-adrenergic blocking agents (propranolol) and quinidine similarly enhance the specific binding of TSH to the guinea pig fat cell membranes over the concentration range of 2 X 10(-4) to 4 X 10(-3) M, increasing the binding affinity of TSH to the membranes. The percentage bound increased from 8% in the absence of agents to 32% (phentolamine), 29% (propranolol) and 24% (quinidine), respectively in the presence of these agents (1.5 X 10(-3) M). Each minimal detectable quantity of TSH was approximately 10 microU per tube in the presence of these agents (10(-3) M) as compared to 100 microU per tube in their absence. Both phentolamine and propranolol appeared to enhance the TSH binding to fat cell membranes through membrane-active, non-specific effects besides their alpha- and beta-adrenergic blocking activities.

摘要

α-肾上腺素能阻滞剂（酚妥拉明）、β-肾上腺素能阻滞剂（普萘洛尔）和奎尼丁在2×10⁻⁴至4×10⁻³M的浓度范围内，同样能增强促甲状腺激素（TSH）与豚鼠脂肪细胞膜的特异性结合，提高TSH与细胞膜的结合亲和力。结合百分比在无药物时为8%，在存在这些药物（1.5×10⁻³M）时，分别增至32%（酚妥拉明）、29%（普萘洛尔）和24%（奎尼丁）。在存在这些药物（10⁻³M）时，每管可检测到的最低TSH量约为10微单位，而在无药物时为每管100微单位。酚妥拉明和普萘洛尔除了其α-和β-肾上腺素能阻滞活性外，似乎还通过膜活性非特异性作用增强TSH与脂肪细胞膜的结合。