Ostrow R S, McGlennen R C, Shaver M K, Kloster B E, Houser D, Faras A J
Institute of Human Genetics, University of Minnesota Medical School, Minneapolis 55455.
Proc Natl Acad Sci U S A. 1990 Oct;87(20):8170-4. doi: 10.1073/pnas.87.20.8170.
Recently we molecularly cloned and characterized a papillomavirus from a lymph node metastasis of a primary penile carcinoma found in a rhesus monkey; this virus species, rhesus papillomavirus type 1 (RhPV-1), is similar to oncogenic human papillomaviruses (HPVs), such as HPV-16 or HPV-18, in that the RhPV-1 DNA was found to be integrated in the tumor cell DNA. To compare the sexual transmission and oncogenic nature of RhPV-1 with these HPVs, we undertook an extensive retrospective study of a group of rhesus monkeys whose sexual mating and offspring histories were known. These animals had mated directly with the index male mentioned above or were secondarily exposed to this virus through intermediate sexual partners. This study combines cytological, histopathological, and several complementary hybridization and DNA amplification techniques on multiple tissue samples to demonstrate the sexually transmitted nature of RhPV-1. The oncogenic potential of RhPV-1 is suggested in several of the infected animals by the presence of various degrees of neoplasia including squamous cell cancer of the cervix.
最近，我们从一只恒河猴的原发性阴茎癌淋巴结转移灶中对一种乳头瘤病毒进行了分子克隆和特性分析；这种病毒物种，即恒河猴乳头瘤病毒1型（RhPV-1），与致癌性人乳头瘤病毒（HPVs），如HPV-16或HPV-18相似，因为发现RhPV-1 DNA整合在肿瘤细胞DNA中。为了比较RhPV-1与这些HPV的性传播和致癌特性，我们对一组已知性交配和后代历史的恒河猴进行了广泛的回顾性研究。这些动物要么直接与上述索引雄性交配，要么通过中间性伴侣间接接触这种病毒。这项研究结合了细胞学、组织病理学以及多种互补杂交和DNA扩增技术，对多个组织样本进行分析，以证明RhPV-1的性传播特性。在几只受感染的动物中，通过存在包括宫颈癌在内的各种程度的肿瘤形成，提示了RhPV-1的致癌潜力。
