钙离子载体A23187可诱导巨噬细胞释放5-脂氧合酶和环氧化酶产物发生双重变化。

Calcium-ionophore A23187 induces dual changes in the release of 5-lipoxygenase and cyclooxygenase products by macrophages.

作者信息

van Hilten J A, Ben Efraim S, Bonta I L

机构信息

Institute of Pharmacology, Erasmus University Rotterdam, Faculty of Medicine, The Netherlands.

出版信息

Arch Int Pharmacodyn Ther. 1990 Mar-Apr;304:55-63.

PMID:2173505

Abstract

A23187-treated murine peritoneal macrophages release increased quantities of the immunoreactive 5-lipoxygenase metabolite leukotriene B4 (LTB4) and the immunoreactive cyclooxygenase products 6-keto prostaglandin F1 alpha (6-keto PGF1 alpha) and thromboxane B2 (TXB2) during a 40 min incubation period. The increase in release of LTB4 was marked already after 5 min of incubation and was maximal after 20 min. The increase in release of 6-keto PGF1 alpha and TXB2 started in most cases after 5 min of incubation and augmented gradually up to 40 min after incubation. The ratio of the increase of LTB4/6-keto PGF1 alpha and of LTB4/TXB2 revealed an increase in favor of LTB4 in the first 5 min of incubation.

摘要

在40分钟的孵育期内，用A23187处理的小鼠腹腔巨噬细胞释放出更多的免疫反应性5-脂氧合酶代谢产物白三烯B4（LTB4）以及免疫反应性环氧化酶产物6-酮前列腺素F1α（6-酮PGF1α）和血栓素B2（TXB2）。孵育5分钟后，LTB4的释放增加就已很明显，20分钟时达到最大值。6-酮PGF1α和TXB2的释放增加在大多数情况下在孵育5分钟后开始，并在孵育后逐渐增加直至40分钟。LTB4/6-酮PGF1α和LTB4/TXB2的增加比例显示，在孵育的前5分钟，LTB4的增加更为明显。