Institute of Otorhinolaryngology, Catholic University of Medicine and Surgery, Rome, Italy.
Rhinology. 2011 Jun;49(2):174-9. doi: 10.4193/Rhino10.133.
The aim of our study was to measure CCL24 (eotaxin-2) levels in nasal lavage fluid of patients with different forms of sinonasal chronic eosinophilic inflammation to verify the relationship with nasal hypereosinophilia and symptoms.
Patients with nasal hypereosinophilia were randomly recruited and grouped in persistent allergic rhinitis, non-allergic rhinitis with eosinophilia syndrome (NARES) and chronic rhinosinusitis with polyps. Non rhinitic volunteers were recruited as controls. CCL24 concentration was measured by Quantikine Human CCL24 Immunoassay. Differential cell counts were performed by microscopic cytological examination of nasal tissue scraped by inferior turbinate.
CCL24 levels measured in patient groups were significantly higher compared to control group with the highest levels in NARES patients. Eotaxin- 2 levels were significantly correlated to severity of symptoms and to the percentage of eosinophils in nasal tissue.
We revealed high levels of CCL24 in all patient groups showing a significant correlation with the degree of eosinophilia and clinical symptoms. A prolonged accumulation of CCL24 inside the nasal mucosa may sustain the process of unspecific self-perpetuating eosinophil recruitment pathognomonic of these patients.
我们的研究旨在测量不同类型鼻-鼻窦慢性嗜酸性粒细胞炎症患者鼻洗液中 CCL24（嗜酸性粒细胞趋化因子-2）的水平，以验证其与鼻嗜酸性粒细胞增多和症状的关系。
我们随机招募了伴有鼻嗜酸性粒细胞增多的患者，并将其分为持续性变应性鼻炎、非变应性嗜酸性粒细胞增多综合征（NARES）和鼻息肉型慢性鼻-鼻窦炎。招募非变应性志愿者作为对照组。通过对下鼻甲刮取的鼻组织进行显微镜细胞化学检查，测量 CCL24 浓度。
与对照组相比，患者组的 CCL24 水平明显升高，其中 NARES 患者的水平最高。嗜酸性粒细胞趋化因子-2 水平与症状严重程度和鼻组织中嗜酸性粒细胞的百分比呈显著相关。
我们发现所有患者组的 CCL24 水平均较高，与嗜酸性粒细胞增多和临床症状有显著相关性。CCL24 在鼻黏膜内的长期积聚可能会持续维持这些患者特有的非特异性自我延续性嗜酸性粒细胞募集过程。
