应用高效液相色谱法检测西班牙市场干无花果中的赭曲霉毒素 A。
The use of high-performance liquid chromatography to detect ochratoxin A in dried figs from the Spanish market.
机构信息
Departamento de Nutrición, Bromatología y Tecnología de Alimentos. Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
出版信息
J Sci Food Agric. 2012 Jan 15;92(1):74-7. doi: 10.1002/jsfa.4543. Epub 2011 Jul 11.
BACKGROUND
Detection and quantification of ochratoxin A (OTA) in dried fig samples purchased in Spain has been carried out using high-performance liquid chromatography with fluorescence detection after extraction with methanol and sodium bicarbonate, and clean-up by using an immunoaffinity column.
RESULTS
The detection limit of the method was 0.06 ng g(-1), and the limit of quantification 0.18 ng g(-1) . OTA was detected in 31 (88.6%) out of 35 samples of dried figs analysed, with concentrations that ranged from < 0.1 to 277 ng g(-1). However, only three samples contained OTA concentrations above the tolerable level set by European Commission regulations for dried vine fruits (10 ng g(-1)).
CONCLUSION
The results of this survey show the value of monitoring OTA in dried figs especially if they are home grown.
背景
使用高效液相色谱法（HPLC）结合荧光检测，从经甲醇和碳酸氢钠提取、免疫亲和柱净化的干无花果样品中检测和定量赭曲霉毒素 A（OTA）。
结果
该方法的检测限为 0.06 纳克/克，定量限为 0.18 纳克/克。对 35 份干无花果样品进行分析，其中 31 份（88.6%）检测到 OTA，浓度范围为<0.1 至 277 纳克/克。然而，只有三个样本中 OTA 浓度超过欧盟委员会法规规定的干葡萄果（10 纳克/克）可接受水平。
结论
这项调查结果表明，特别是如果是自家种植的，监测干无花果中 OTA 的含量是有价值的。
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