Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, IL 60637, USA.
Angew Chem Int Ed Engl. 2011 Aug 22;50(35):8029-33. doi: 10.1002/anie.201103237. Epub 2011 Jul 8.
Efficient access: the 204-residue covalent-dimer vascular endothelial growth factor with full mitogenic activity was prepared from three unprotected peptide segments by one-pot native chemical ligations. The covalent structure of the synthetic VEGF was confirmed by precise mass measurement, and the three-dimensional structure of the synthetic protein was determined by high-resolution X-ray crystallography.
全长 204 个残基的具有完全有丝分裂活性的共价二聚体血管内皮生长因子由三个未保护的肽段通过一锅法的天然化学连接制备而成。合成 VEGF 的共价结构通过精确质量测量得到证实,其三维结构通过高分辨率 X 射线晶体学确定。