多中心评估一种新的 DNA 微阵列，用于快速检测来自β-内酰胺类耐药革兰氏阴性菌的临床相关 bla 基因。
Multicenter evaluation of a new DNA microarray for rapid detection of clinically relevant bla genes from beta-lactam-resistant gram-negative bacteria.
机构信息
Laboratoire de Bactériologie, Cliniques Universitaires UCL de Mont-Godinne, Av Dr Therasse 1, B-5530 Yvoir, Belgium.
出版信息
Antimicrob Agents Chemother. 2011 Sep;55(9):4457-60. doi: 10.1128/AAC.00353-11. Epub 2011 Jul 11.
Abstract
A new commercial low-density microarray which identifies common extended-spectrum β-lactamase plasmid-mediated cephalosporinase genes, as well as carbapenemase (bla(KPC) and bla(NDM)) genes, was evaluated. We tested 207 clinical and reference/collection isolates of the Enterobacteriaceae possessing different bla genes. Overall, the sensitivity and specificity of the microarray were 100% for the detection of the plasmid-mediated bla(AmpC), bla(KPC), and bla(NDM) genes using bla gene sequencing as the reference method.
摘要
我们评估了一种新的商用低密度微阵列，该微阵列可识别常见的扩展谱β-内酰胺酶质粒介导的头孢菌素酶基因，以及碳青霉烯酶（bla(KPC)和 bla(NDM))基因。我们测试了 207 株具有不同 bla 基因的肠杆菌科临床和参考/收集分离株。总体而言，使用 bla 基因测序作为参考方法，该微阵列检测质粒介导的 bla(AmpC)、bla(KPC)和 bla(NDM)基因的灵敏度和特异性均为 100%。
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