秋水仙碱处理原代培养胎鼠肝细胞后细胞修复过程中碱性磷酸酶和组织蛋白酶 D 的定位。

Localization of Alkaline Phosphatase and Cathepsin D during Cell Restoration after Colchicine Treatment in Primary Cultures of Fetal Rat Hepatocytes.

机构信息

Department of Anatomy, School of Allied Health Sciences, Kitasato University, Sagamihara, Kanagawa 228-8555, Japan.

出版信息

Acta Histochem Cytochem. 2011 Jun 29;44(3):155-8. doi: 10.1267/ahc.10037. Epub 2011 Jun 3.

DOI:10.1267/ahc.10037

PMID:21753861

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3130147/

Abstract

Localization of alkaline phosphatase (ALP) and cathepsin D (CAPD) in primary cultures of fetal rat hepatocytes was examined using double immunofluorescent staining in order to investigate the relationship between lysosome movement and the fate of ALP during cell restoration after microtubule disruption by colchicine. At 3 hr and 24 hr after colchicine treatment, numerous coarse dots containing ALP were observed throughout the cytoplasm, and some of these showed colocalization with CAPD. At 48 hr and 72 hr after colchicine treatment, although most of the dots containing ALP in the cytoplasm disappeared, dots containing CAPD remained. The present results suggest that the denatured ALP proteins remaining in the cytoplasm of hepatocytes during cell restoration after colchicine treatment are digested by lysosomes.

摘要

为了研究微管解聚药物秋水仙素处理后细胞恢复过程中溶酶体运动与碱性磷酸酶（ALP）命运之间的关系，我们利用双重免疫荧光染色技术对原代培养的胎鼠肝细胞中碱性磷酸酶（ALP）和组织蛋白酶 D（CAPD）进行了定位研究。秋水仙素处理后 3 小时和 24 小时，细胞质中观察到大量含有 ALP 的粗斑点，其中一些与 CAPD 共定位。秋水仙素处理后 48 小时和 72 小时，虽然细胞质中含有 ALP 的大多数斑点消失，但含有 CAPD 的斑点仍然存在。这些结果表明，在秋水仙素处理后细胞恢复过程中，细胞质中残留的变性 ALP 蛋白被溶酶体消化。