Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark.
Ticks Tick Borne Dis. 2010 Jun;1(2):101-3. doi: 10.1016/j.ttbdis.2010.01.004. Epub 2010 Feb 4.
A total of 704 unfed ticks of the species Ixodes ricinus collected in Denmark were screened for Rickettsia DNA by a genus-specific real-time PCR. Of the nymphs, 4.7% (31/662) were positive for rickettsial DNA. Among the positive ticks, we observed a seasonal and habitat variation. The infection rate was highest in May as compared to July, August, and October. Ecotone (high tick density) showed an elevated prevalence as compared to spruce or beech forests. Sequencing revealed only DNA from R. helvetica.
丹麦共采集了 704 只未进食的蓖子硬蜱,通过种特异性实时 PCR 对其进行了立克次体 DNA 筛查。在若虫中,有 4.7%(31/662)的蜱虫对立克次体 DNA 呈阳性。在所观察到的阳性蜱虫中,存在季节性和生境差异。与 7 月、8 月和 10 月相比,5 月的感染率最高。与云杉或山毛榉林相比,生态过渡带(高蜱密度)的流行率更高。测序仅显示出从 R. helvetica 分离的 DNA。