A triazine bridged p-phenylenevinylene polymer film for biomolecular patterning.

Photo-reaction by UV irradiation of a highly fluorescent s-triazine bridged p-phenylenevinylene polymer resulted in micro and submicron fluorescent pattern because carbonyl group (C=O) was generated from vinylene group (C=C) through the photo-oxidation. This fluorescent pattern could be used for micro scale cell patterning as well as submicron scale biomolecules patterning such as proteins. When exposed to a solution containing biomolecules, the polymeric patterns were selectively coated with biomoleucles, to result in biomolecular patterns. In particular, the UV exposed area of the poly[4,6-bis(phenoxy)-2-diphenylamino-s-triazine]co(2, 5-bis(trimethylsily)-1,4-phenylenevinylene) (DTSPV) patterns was highly selective toward fluorescein isothiocyanate (FITC) conjugated-collagen. These studies provide an exciting opportunity for tissue engineering and fundamental understanding of the mechanisms of cellular adhesion, proliferation, and differentiation.