大亚基核糖体 DNA 的 D1/D2 结构域可用于区分 Orpinomyces spp.
D1/D2 domain of large-subunit ribosomal DNA for differentiation of Orpinomyces spp.
机构信息
Dairy Microbiology Division, National Dairy Research Institute, Karnal, India.
出版信息
Appl Environ Microbiol. 2011 Sep;77(18):6722-5. doi: 10.1128/AEM.05441-11. Epub 2011 Jul 22.
Abstract
This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.
摘要
本研究提出了基于 PCR-限制性片段长度多态性 (RFLP) 的大亚基 (LSU) 核糖体 DNA (rDNA) 的 D1/D2 结构域区分琼氏嗜黏蛋白菌和中间嗜黏蛋白菌的适用性。在中间嗜黏蛋白菌中 G/T 的变异产生了一个额外的 AluI 限制性位点，可作为 RFLP 标记。结果表明 LSU rDNA 具有足够的种间异质性用于物种水平的区分。
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