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东方白鹳(Ciconia boyciana)微卫星DNA标记的分离与鉴定

Isolation and characterization of microsatellite DNA markers for the Oriental White stork, Ciconia boyciana.

作者信息

Wang Hui, Luo Xia, Zhu Qiongqiong, Huang Yun, Zhou Lizhi, Zhang Baowei

机构信息

School of Life Sciences, Anhui University, Hefei 230039, Anhui, China.

出版信息

Zoolog Sci. 2011 Aug;28(8):606-8. doi: 10.2108/zsj.28.606.

Abstract

Following FIASCO protocol and across-amplification approach, we present eleven microsatellite primers of the Oriental White stork, Ciconia boyciana in this article. All loci were polymorphic, except for locus Cbo235, which possessed two alleles but was homozygous in all 23 individuals of C. boyciana used in this study. The number of the alleles per locus ranged from two to eight, and the observed heterozygosity (H(o)) and expected heterozygosity (H(E)) ranged from 0 to 0.857 and 0.222 to 0.851, respectively. These markers are proved useful in genetic study of C. boyciana.

摘要

遵循FIASCO方案和跨扩增方法,我们在本文中展示了东方白鹳(Ciconia boyciana)的11个微卫星引物。所有位点均为多态性,除了位点Cbo235,该位点有两个等位基因,但在本研究中使用的所有23只东方白鹳个体中均为纯合子。每个位点的等位基因数量从2到8不等,观察到的杂合度(H(o))和预期杂合度(H(E))分别从0到0.857和0.222到0.851不等。这些标记物被证明在东方白鹳的遗传学研究中有用。

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