[高效液相色谱法同时测定凌霄花中紫丁香苷、齐墩果酸和熊果酸的含量]
[Simultaneous determination of acteoside, oleanolic acid and ursolic acid in flower of Campsis grandiflora by HPLC].
作者信息
Zhang Qiao, Shen Juan, Liu Yujie, Bi Yu'an, Wang Zhenzhong, Xiao Wei
机构信息
Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Apr;36(8):1043-5.
OBJECTIVE
To develop an HPLC method for the determination of acteoside, oleanolic acid and ursolic acid in flowers of Campsis grandiflora.
METHOD
The analysis was carried out on an Agilent ZORBAX Eclipse XDB-18 column eluted with methanol and 0.1% phosphoric acid in gradient mode. The detection wavelength was set at 334 mn at 0-30 min and 210 nm at 30-60 min.
RESULT
The peak areas and concentrations have a good linear relationship at 0.025 9-0.258 g x L(-1) for acteoside, 0.100-1.00 g x L(-1) for oleanolic acid and 0.104-1.04 g x L(-1) for ursolic acid, respectively. The average recoveries were 98.9%, 99.3% and 99.40%, respectively.
CONCLUSION
The method can determinate the concentration of acteoside, oleanolic acid and ursolic acid simultaneously. It can be used for the quality control of flower of C. grandiflora.
目的
建立一种高效液相色谱法测定凌霄花中毛蕊花糖苷、齐墩果酸和熊果酸的含量。
方法
采用安捷伦ZORBAX Eclipse XDB - 18色谱柱，以甲醇和0.1%磷酸为流动相进行梯度洗脱。检测波长在0 - 30分钟时设定为334nm，30 - 60分钟时设定为210nm。
结果
毛蕊花糖苷在0.025 9 - 0.258 g·L⁻¹、齐墩果酸在0.100 - 1.00 g·L⁻¹、熊果酸在0.104 - 1.04 g·L⁻¹范围内，峰面积与浓度分别具有良好的线性关系。平均回收率分别为98.9%、99.3%和99.40%。
结论
该方法可同时测定凌霄花中毛蕊花糖苷、齐墩果酸和熊果酸的含量，可用于凌霄花的质量控制。
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