支原体和衣原体属之间的序列同源性导致用商业 PCR 检测法检测支原体污染的衣原体细胞培养物时出现假阳性结果。
Sequence homologies between Mycoplasma and Chlamydia spp. lead to false-positive results in chlamydial cell cultures tested for mycoplasma contamination with a commercial PCR assay.
机构信息
Institute of Medical Microbiology, Hygiene and Infectious Diseases, PMU University Hospital Salzburg, Müllner Hauptstr. 48, 5020 Salzburg, Austria.
出版信息
J Clin Microbiol. 2011 Oct;49(10):3681-2. doi: 10.1128/JCM.01092-11. Epub 2011 Aug 17.
Abstract
Mycoplasma contamination is a frequent problem in chlamydial cell culture. After obtaining contradictory contamination results, we compared three commercial PCR kits for mycoplasma detection. One kit signaled contamination in mycoplasma-free Chlamydia pneumoniae cultures. Sequencing of cloned PCR products revealed primer homology with the chlamydial genome as the basis of this false-positive result.
摘要
支原体污染是衣原体细胞培养中常见的问题。在获得相互矛盾的污染结果后，我们比较了三种用于支原体检测的商业 PCR 试剂盒。一种试剂盒在无支原体的肺炎衣原体培养物中发出了污染信号。克隆 PCR 产物的测序显示，引物与衣原体基因组的同源性是这种假阳性结果的基础。
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