血红素/G-四链体作为 DNA 酶用于荧光检测 DNA、适配体-凝血酶复合物，并探测葡萄糖氧化酶的活性。
Hemin/G-quadruplexes as DNAzymes for the fluorescent detection of DNA, aptamer-thrombin complexes, and probing the activity of glucose oxidase.
机构信息
Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
出版信息
Analyst. 2011 Nov 7;136(21):4397-401. doi: 10.1039/c1an15596b. Epub 2011 Sep 1.
PMID:21881641
Abstract
Hemin/G-quadruplex catalyzes the H(2)O(2)-mediated oxidation of Amplex Red to the fluorescent product resorufin. This process is implemented to develop hairpin nucleic acid structures for the detection of DNA, to probe the catalytic activity of glucose oxidase, to use the thrombin-aptamer complex as a catalytic readout structure, and to quantitatively analyze telomere chain composition.
摘要
血红素/G-四链体催化 H(2)O(2)介导的 Amplex Red 氧化生成荧光产物 Resorufin。该过程用于开发发夹核酸结构以检测 DNA，探测葡萄糖氧化酶的催化活性，使用凝血酶适配体复合物作为催化读出结构，以及定量分析端粒链组成。
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