Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina, USA.
Nat Protoc. 2011 Aug 18;6(9):1355-66. doi: 10.1038/nprot.2011.349.
Optical techniques for functional imaging in mice have a number of key advantages over other common imaging modalities such as magnetic resonance imaging, positron emission tomography or computed tomography, including high resolution, low cost and an extensive library of available contrast agents and reporter genes. A major challenge to such work is the limited penetration depth imposed by tissue turbidity. We describe a window chamber technique by which these limitations can be avoided. This facilitates the study of a wide range of processes, with potential endpoints including longitudinal gene expression, vascular remodeling and angiogenesis, and tumor growth and invasion. We further describe several quantitative imaging and analysis techniques for characterizing in vivo fluorescence properties and functional endpoints, including vascular morphology and oxygenation. The procedure takes ∼2 h to complete, plus up to several weeks for tumor growth and treatment procedures.
光学技术在小鼠功能成像方面具有许多优于磁共振成像、正电子发射断层扫描或计算机断层扫描等常见成像方式的关键优势，包括高分辨率、低成本以及广泛的可用对比剂和报告基因库。这类工作的一个主要挑战是组织浑浊造成的有限穿透深度。我们描述了一种窗室技术，可以避免这些限制。这有利于研究广泛的过程，潜在的终点包括纵向基因表达、血管重塑和血管生成以及肿瘤生长和侵袭。我们还进一步描述了几种定量成像和分析技术，用于描述体内荧光特性和功能终点，包括血管形态和氧合作用。该过程大约需要 2 小时才能完成，再加上肿瘤生长和治疗过程可能需要数周时间。
