腹泻型肉鸡中肠毒素型大肠杆菌的检测

Demonstration of enterotoxigenic Escherichia coli in diarrheic broiler chicks.

作者信息

Joya J E, Tsuji T, Jacalne A V, Arita M, Tsukamoto T, Honda T, Miwatani T

机构信息

Research Institute for Microbial Disease, Osaka University, Japan.

出版信息

Eur J Epidemiol. 1990 Mar;6(1):88-90. doi: 10.1007/BF00155557.

DOI:10.1007/BF00155557

PMID:2188851

Abstract

An investigation was made to survey the possible presence of enterotoxigenic Escherichia coli (ETEC) in the stools of diarrheal chicks. We analyzed two outbreaks of diarrhea in broiler chicks at two independent farms in the Philippines, from which no pathogens other than Escherichia coli were found. In one outbreak at Farm #1, all 42 isolates produced heat-labile enterotoxin (LT), with 3 of these isolates also producing heat-stable enterotoxin (ST). The O serotypes of 15 strains tested randomly could not be identified as any known serotype (0-antigen; 1-170). In another outbreak at Farm #2, 7 out of 52 isolates produced only LT, their subtypes being identified as O-149 or O-8, common serotypes in pig ETEC. Strains from Farm #1 did not produce any pili usually found in human ETEC. We believe this to be the first isolation of ETEC from diarrheal chicks.

摘要

对腹泻雏鸡粪便中是否存在产肠毒素大肠杆菌（ETEC）进行了调查。我们分析了菲律宾两个独立农场肉鸡雏鸡的两起腹泻疫情，除大肠杆菌外未发现其他病原体。在农场1的一次疫情中，所有42株分离株均产生不耐热肠毒素（LT），其中3株还产生耐热肠毒素（ST）。随机检测的15个菌株的O血清型无法鉴定为任何已知血清型（O抗原；1 - 170）。在农场2的另一次疫情中，52株分离株中有7株仅产生LT，其亚型被鉴定为O - 149或O - 8，这是猪ETEC中的常见血清型。农场1的菌株未产生通常在人ETEC中发现的任何菌毛。我们认为这是首次从腹泻雏鸡中分离出ETEC。