Department of Polymer Science and Engineering, Kyungpook National University, Daegu 702-701, South Korea.
Nanoscale Res Lett. 2011 Sep 23;6(1):528. doi: 10.1186/1556-276X-6-528.
Cytotoxicity is a severe problem for cadmium sulfide nanoparticles (CSNPs) in biological systems. In this study, mercaptoacetic acid-coated CSNPs, typical semiconductor Q-dots, were synthesized in aqueous medium by the arrested precipitation method. Then, amino-terminated polyethylene glycol (PEG) was conjugated to the surface of CSNPs (PCSNPs) in order to introduce amino groups to the surface. Finally, insulin was immobilized on the surface of PCSNPs (ICSNPs) to reduce cytotoxicity as well as to enhance cell compatibility. The presence of insulin on the surface of ICSNPs was confirmed by observing infrared absorptions of amide I and II. The mean diameter of ICSNPs as determined by dynamic light scattering was about 38 nm. Human fibroblasts were cultured in the absence and presence of cadmium sulfide nanoparticles to evaluate cytotoxicity and cell compatibility. The results showed that the cytotoxicity of insulin-immobilized cadmium sulfide nanoparticles was significantly suppressed by usage of PEG as a spacer. In addition, cell proliferation was highly facilitated by the addition of ICSNPs. The ICSNPs used in this study will be potentials to be used in bio-imaging applications.
细胞毒性是生物体系中硫化镉纳米粒子（CSNPs）的一个严重问题。在本研究中，采用停止沉淀法在水相介质中合成了巯基乙酸包覆的 CSNPs，这是典型的半导体 Q 点。然后，将氨端聚乙二醇（PEG）接枝到 CSNPs 表面（PCSNPs），以在表面引入氨基。最后，胰岛素被固定在 PCSNPs（ICSNPs）表面，以降低细胞毒性并提高细胞相容性。通过观察酰胺 I 和 II 的红外吸收，证实了胰岛素存在于 ICSNPs 的表面。通过动态光散射测定的 ICSNPs 的平均粒径约为 38nm。用人成纤维细胞在有无硫化镉纳米粒子的情况下培养，以评估细胞毒性和细胞相容性。结果表明，使用 PEG 作为间隔物可以显著抑制胰岛素固定化硫化镉纳米粒子的细胞毒性。此外，添加 ICSNPs 可高度促进细胞增殖。本研究中使用的 ICSNPs 将有可能用于生物成像应用。
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