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评估EZ-SCREEN酶免疫分析试验用于检测尿样中可卡因和大麻代谢物的情况。

Evaluation of the EZ-SCREEN enzyme immunoassay test for detection of cocaine and marijuana metabolites in urine specimens.

作者信息

Schwartz R H, Bogema S, Thorne M M

机构信息

Department of Pediatrics, Fairfax Hospital, Falls Church, Virginia.

出版信息

Pediatr Emerg Care. 1990 Jun;6(2):147-9. doi: 10.1097/00006565-199006000-00022.

DOI:10.1097/00006565-199006000-00022
PMID:2196539
Abstract

The EZ-SCREEN Cannabinoid/Cocaine test is a qualitative enzyme immunoassay card test for the detection of cannabinoid and cocaine metabolites in urine specimens. Results are available in about three minutes. The test kit costs approximately $12.00 per two-drug test, and there are no additional expenses for analytical equipment. We compared the results of the EZ-SCREEN test with results of specimens analyzed by gas chromatography/mass spectrometry (GC/MS) for 36 clinical specimens positive for cannabinoids (marijuana), 38 clinical specimens positive for benzoylecgonine (cocaine), and 33 drug-free specimens. Results from our study for marijuana included: sensitivity = 92%; specificity = 89%; and efficiency = 95, 67, and 87%, respectively. In the present study, there were five false-positive tests for cocaine and one false-positive test for cannabinoids. As with all screening test results, confirmation of positive specimens by a more specific quantitative analytical method such as GC/MS is recommended.

摘要

EZ-SCREEN大麻素/可卡因检测是一种用于检测尿液样本中大麻素和可卡因代谢物的定性酶免疫分析卡式检测。约三分钟即可出结果。该检测试剂盒每进行一次双药检测的成本约为12美元,且无需额外的分析设备费用。我们将EZ-SCREEN检测结果与通过气相色谱/质谱联用仪(GC/MS)分析的36份大麻素(大麻)阳性临床样本、38份苯甲酰爱康宁(可卡因)阳性临床样本以及33份无毒品样本的检测结果进行了比较。我们对大麻的研究结果包括:灵敏度=92%;特异性=89%;以及效率分别为95%、67%和87%。在本研究中,可卡因检测有5例假阳性,大麻素检测有1例假阳性。与所有筛查检测结果一样,建议通过更具特异性的定量分析方法(如GC/MS)对阳性样本进行确认。

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