Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao 266003, China.
Mar Biotechnol (NY). 2012 Apr;14(2):245-51. doi: 10.1007/s10126-011-9408-1. Epub 2011 Oct 14.
Sulfide is a natural, widely distributed, poisonous substance, and sulfide:quinone oxidoreductase (SQR) is responsible for the initial oxidation of sulfide in mitochondria. In this study, we examined the response of SQR to sulfide exposure (25, 50, and 150 μM) at mRNA, protein, and enzyme activity levels in the body wall and hindgut of the echiuran worm Urechis unicinctus, a benthic organism living in marine sediments. The results revealed SQR mRNA expression during sulfide exposure in the body wall and hindgut increased in a time- and concentration-dependent manner that increased significantly at 12 h and continuously increased with time. At the protein level, SQR expression in the two tissues showed a time-dependent relationship that increased significantly at 12 h in 50 μM sulfide and 6 h in 150 μM, and then continued to increase with time while no significant increase appeared after 25 μM sulfide exposure. SQR enzyme activity in both tissues increased significantly in a time-dependent manner after 50 μM sulfide exposure. We concluded that SQR expression could be induced by sulfide exposure and that the two tissues studied have dissimilar sulfide metabolic patterns. A U. unicinctus sulfide-induced detoxification mechanism was also discussed.
硫化物是一种天然、广泛分布的有毒物质，而硫化物：醌氧化还原酶（SQR）负责在线粒体中最初氧化硫化物。在这项研究中，我们检查了 SQR 对贻虫 Urechis unicinctus 体壁和后肠中硫化物暴露（25、50 和 150μM）的反应，贻虫是一种生活在海洋沉积物中的底栖生物。结果表明，在体壁和后肠中，SQR mRNA 的表达在暴露于硫化物时呈时间和浓度依赖性增加，在 12 小时时显著增加，并随时间持续增加。在蛋白质水平上，两种组织中的 SQR 表达呈时间依赖性关系，在 50μM 硫化物中 12 小时显著增加，在 150μM 中 6 小时显著增加，然后随时间继续增加，而在 25μM 硫化物暴露后没有明显增加。两种组织中的 SQR 酶活性在 50μM 硫化物暴露后均呈时间依赖性显著增加。我们得出结论，SQR 的表达可以被硫化物暴露诱导，并且所研究的两种组织具有不同的硫化物代谢模式。还讨论了贻虫的硫化物诱导解毒机制。
