Dashtban Mehdi, Buchkowski Robert, Qin Wensheng
Int J Biochem Mol Biol. 2011;2(3):274-86. Epub 2011 Sep 9.
The ascomycete Hypocrea jecorina, an industrial (hemi)cellulase producer, can efficiently degrade plant polysaccharides. At present, the biology underlying cellulase hyperproduction of T. reesei, and the conditions for the enzyme induction, are not completely understood. In the current study, three different strains of T. reesei, including QM6a (wild-type), and mutants QM9414 and RUT-C30, were grown on 7 soluble and 7 insoluble carbon sources, with the later group including 4 pure polysaccharides and 3 lignocelluloses. Time course experiments showed that maximum cellulase activity of QM6a and QM9414 strains, for the majority of tested carbon sources, occurred at 120 hrs, while RUT-C30 had the greatest cellulase activity around 72 hrs. Maximum cellulase production was observed to be 0.035, 0.42 and 0.33 µmol glucose equivalents using microcrystalline celluloses for QM6a, QM9414, and RUTC-30, respectively. Increased cellulase production was positively correlated in QM9414 and negatively correlated in RUT-C30 with ability to grow on microcrystalline cellulose.
子囊菌里氏木霉是一种工业用（半）纤维素酶产生菌，能够高效降解植物多糖。目前，里氏木霉纤维素酶高产的生物学机制以及酶诱导条件尚未完全明确。在本研究中，使用7种可溶性碳源和7种不溶性碳源培养了三种不同的里氏木霉菌株，包括QM6a（野生型）以及突变体QM9414和RUT - C30，后者包括4种纯多糖和3种木质纤维素。时间进程实验表明，对于大多数测试碳源，QM6a和QM9414菌株的最大纤维素酶活性出现在120小时，而RUT - C30在72小时左右具有最大纤维素酶活性。使用微晶纤维素时，QM6a、QM9414和RUT - C30的最大纤维素酶产量分别为0.035、0.42和0.33微摩尔葡萄糖当量。QM9414中纤维素酶产量的增加与在微晶纤维素上生长的能力呈正相关，而在RUT - C30中呈负相关。
