缺失 iclR 和 arcA 对大肠杆菌 BL21 (DE3) 的生理和代谢通量的影响。
Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3).
机构信息
Centre of Expertise-Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium.
出版信息
Biotechnol Lett. 2012 Feb;34(2):329-37. doi: 10.1007/s10529-011-0774-6. Epub 2011 Oct 19.
Deletion of both iclR and arcA in E. coli profoundly alters the central metabolic fluxes and decreases acetate excretion by 70%. In this study we investigate the metabolic consequences of both deletions in E. coli BL21 (DE3). No significant differences in biomass yields, acetate yields, CO(2) yields and metabolic fluxes could be observed between the wild type strain E. coli BL21 (DE3) and the double-knockout strain E. coli BL21 (DE3) ΔarcAΔiclR. This proves that arcA and iclR are poorly active in the BL21 wild type strain. Noteworthy, both strains co-assimilate glucose and acetate at high glucose concentrations (10-15 g l(-1)), while this was never observed in K12 strains. This implies that catabolite repression is less intense in BL21 strains compared to in E. coli K12.
缺失 iclR 和 arcA 基因会显著改变大肠杆菌的中心代谢通量，并使乙酸盐的排泄量减少 70%。在这项研究中，我们研究了 E. coli BL21 (DE3) 中这两个缺失的代谢后果。在野生型大肠杆菌 BL21 (DE3) 和双敲除菌株 E. coli BL21 (DE3) ΔarcAΔiclR 之间，生物量产量、乙酸盐产量、CO(2)产量和代谢通量均无显著差异。这证明 arcA 和 iclR 在 BL21 野生型菌株中活性较差。值得注意的是，两种菌株在高葡萄糖浓度（10-15 g l(-1)) 下均可共同同化葡萄糖和乙酸盐，而在 K12 菌株中从未观察到这一现象。这意味着与 E. coli K12 相比，BL21 菌株中的分解代谢物阻遏作用较弱。
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