Wu R H, Wang P, Yang L, Li Y, Liu Y, Liu M
Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, China.
Genet Mol Res. 2011 Oct 21;10(4):3914-23. doi: 10.4238/2011.October.21.7.
Myostatin is a secreted negative regulator of muscle mass, and follistatin antagonizes the function of several members of the TGF-b family, including myostatin. Previously, myostatin expression was found to be closely associated with atrophy of the gastrocnemius muscle, showing a linear correlation, after sciatic nerve injury. In this study, we investigated the possibility of myostatin being an indicator of denervated muscle atrophy. ELISA was used to detect the concentration of myostatin and follistatin in sera collected from individual rats at different times after sciatic nerve crush. A strong correlation was shown between the expression level of secreted myostatin in circulation and the wet weight ratio of the gastrocnemius muscle. The ratio of follistatin/myostatin could be used to monitor the progress of target muscle atrophy and recovery. Our study provides a potential serological test to detect denervated muscle atrophy for clinical purposes.
肌肉生长抑制素是一种分泌型的肌肉质量负调节因子，而卵泡抑素可拮抗包括肌肉生长抑制素在内的转化生长因子-β（TGF-β）家族多个成员的功能。此前研究发现，坐骨神经损伤后，肌肉生长抑制素的表达与腓肠肌萎缩密切相关，呈线性关系。在本研究中，我们探讨了肌肉生长抑制素作为失神经支配肌肉萎缩指标的可能性。采用酶联免疫吸附测定法（ELISA）检测坐骨神经挤压后不同时间点从大鼠个体采集的血清中肌肉生长抑制素和卵泡抑素的浓度。循环中分泌型肌肉生长抑制素的表达水平与腓肠肌湿重比之间呈现出强烈的相关性。卵泡抑素与肌肉生长抑制素的比值可用于监测目标肌肉萎缩和恢复的进程。我们的研究为临床检测失神经支配肌肉萎缩提供了一种潜在的血清学检测方法。
