采用阳离子、疏水和亲水肽的络合和共轭方法评估 siRNA 递呈。
Complexation and conjugation approaches to evaluate siRNA delivery using cationic, hydrophobic and amphiphilic peptides.
机构信息
Department of Chemistry, Division of IBB, Pohang University of Science and Technology, Pohang, 790-784, Republic of Korea.
出版信息
Org Biomol Chem. 2012 Jan 7;10(1):96-102. doi: 10.1039/c1ob06042b. Epub 2011 Nov 1.
PMID:22045520
Abstract
In this study, we used solid phase synthesis to prepare three kinds of peptides and then formulated their peptide-siRNA complexes and peptide-siRNA conjugates. Both the complexation and conjugation systems were nontoxic and allowed the delivery of siRNA into the cytoplasm without the need for any transfection agents and with subsequent inhibition of gene expression.
摘要
在这项研究中，我们使用固相合成法制备了三种肽，然后制备了它们的肽-siRNA 复合物和肽-siRNA 缀合物。复合物和缀合物系统均无毒，能够将 siRNA 递送至细胞质中，而无需任何转染试剂，并随后抑制基因表达。
Zotero 插件