G蛋白偶联受体信号传导的变构机制：结构视角
Allosteric mechanisms of G protein-Coupled Receptor signaling: a structural perspective.
作者信息
Thaker Tarjani M, Kaya Ali I, Preininger Anita M, Hamm Heidi E, Iverson T M
机构信息
Department of Biochemistry, Vanderbilt University Medical Center, Nashville, TN, USA.
出版信息
Methods Mol Biol. 2012;796:133-74. doi: 10.1007/978-1-61779-334-9_8.
Abstract
G protein-Coupled Receptors (GPCRs) use a complex series of intramolecular conformational changes to couple agonist binding to the binding and activation of cognate heterotrimeric G protein (Gαβγ). The mechanisms underlying this long-range activation have been identified using a variety of biochemical and structural approaches and have primarily used visual signal transduction via the GPCR rhodopsin and cognate heterotrimeric G protein transducin (G(t)) as a model system. In this chapter, we review the methods that have revealed allosteric signaling through rhodopsin and transducin. These methods can be applied to a variety of GPCR-mediated signaling pathways.
摘要
G蛋白偶联受体（GPCRs）利用一系列复杂的分子内构象变化，将激动剂结合与同源异源三聚体G蛋白（Gαβγ）的结合和激活偶联起来。这种远程激活的潜在机制已通过多种生化和结构方法得以确定，并且主要以通过GPCR视紫红质和同源异源三聚体G蛋白转导素（G(t)）进行的视觉信号转导作为模型系统。在本章中，我们回顾了揭示通过视紫红质和转导素进行变构信号传导的方法。这些方法可应用于多种GPCR介导的信号通路。
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