基于流感病毒报告细胞的新型病毒抑制剂筛选。
Identification of novel virus inhibitors by influenza A virus specific reporter cell based screening.
机构信息
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
出版信息
Antiviral Res. 2012 Jan;93(1):48-54. doi: 10.1016/j.antiviral.2011.10.014. Epub 2011 Oct 25.
As influenza viruses have developed resistance towards current drugs, it is urgent to find potential novel antiviral inhibitors. Here we generated an influenza virus reporter cell line in which the luciferase gene was driven by the influenza virus promoter and screened a small compound library (NCI Diversity Set II). Ten compounds were identified to have inhibitory activity against influenza A virus H1N1. Among them, four compounds blocked influenza virus replication through inhibiting the activity of vRNP. The compound NSC 335506 inhibited HA-mediated membrane fusion. It showed the inhibitory activity against H1N1, H9N2 and H5N1 subtype but not H3N2. Our results demonstrated that influenza virus reporter cell is a very useful tool to identify novel inhibitors against influenza A virus.
由于流感病毒对现有药物产生了耐药性，因此急需寻找潜在的新型抗病毒抑制剂。在这里，我们构建了一个流感病毒报告细胞系，该细胞系中的荧光素酶基因由流感病毒启动子驱动，并筛选了一个小型化合物文库（NCI 多样性集 II）。有 10 种化合物被鉴定对甲型流感病毒 H1N1 具有抑制活性。其中，有 4 种化合物通过抑制 vRNP 的活性来阻断流感病毒的复制。化合物 NSC 335506 抑制了 HA 介导的膜融合。它对 H1N1、H9N2 和 H5N1 亚型均有抑制活性，但对 H3N2 亚型没有抑制活性。我们的研究结果表明，流感病毒报告细胞系是一种非常有用的工具，可用于鉴定抗甲型流感病毒的新型抑制剂。
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