Thurlow P J, Kenneally D A, Connellan J M
Department of Haematology, Austin Hospital, Heidelberg, Victoria, Australia.
Br J Haematol. 1990 Aug;75(4):549-56. doi: 10.1111/j.1365-2141.1990.tb07797.x.
A monoclonal antibody (anti-Fn2) was prepared which was reactive with both plasma fibronectin and fibronectin located within the platelet alpha granule. Immunoblotting analysis, on thermolysin digestion fragments of fibronectin, identified two immunoreactive fragments of Mr 145 kDa and 155 kDa which are known to contain a cell and DNA binding region. Anti-Fn2 was found to inhibit binding of fibronectin to platelets and DNA. Functional platelet studies, measuring platelet aggregation and 14C-serotonin release in washed platelet systems, demonstrated the ability of anti-Fn2 to totally inhibit low dose thrombin and low-dose collagen induced platelet aggregation and serotonin release. Anti-Fn2 partially inhibited platelet aggregation induced by ADP (10 microM) and arachidonic acid, but had no effect on platelet aggregation induced by high-dose thrombin or by the calcium ionophore A23187. These studies indicate that fibronectin participates in platelet aggregation and release induced by a range of agonists and suggest that it has a more important involvement in platelet function than previously described.
制备了一种单克隆抗体（抗Fn2），它能与血浆纤连蛋白以及血小板α颗粒内的纤连蛋白发生反应。对纤连蛋白的嗜热菌蛋白酶消化片段进行免疫印迹分析，鉴定出两个免疫反应性片段，分子量分别为145 kDa和155 kDa，已知它们含有细胞和DNA结合区域。发现抗Fn2可抑制纤连蛋白与血小板及DNA的结合。在洗涤过的血小板系统中进行的功能性血小板研究，测量血小板聚集和14C - 5 -羟色胺释放，结果表明抗Fn2能够完全抑制低剂量凝血酶和低剂量胶原诱导的血小板聚集和5 -羟色胺释放。抗Fn2部分抑制ADP（10微摩尔）和花生四烯酸诱导的血小板聚集，但对高剂量凝血酶或钙离子载体A23187诱导的血小板聚集没有影响。这些研究表明纤连蛋白参与了一系列激动剂诱导的血小板聚集和释放，并提示它在血小板功能中的作用比之前描述的更为重要。
