基于 XJ-160 辛德毕斯样病毒反向遗传系统的研究。
Research on basis of reverse genetics system of a Sindbis-like virus XJ-160.
机构信息
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Viral Disease Control and Prevention, Beijing 100052, China.
出版信息
Virol J. 2011 Nov 14;8:519. doi: 10.1186/1743-422X-8-519.
As a Sindbis-like virus (SINLV), XJ-160 virus was isolated from a pooled sample of Anopheles mosquitoes collected in Xinjiang, China, in 1990. Recombinant plasmid pBR-XJ160 is an infectious full-length cDNA clone of XJ-160 virus, from which rescued virus BR-XJ160 can be obtained by transcription in vitro and transfection. The BR-XJ160 virus raised in BHK-21 cells was indistinguishable from the XJ-160 virus in its biological properties, including its plaque morphology, growth kinetics and suckling mouse neurovirulence. On basis of pBR-XJ160, the effects of substitutions within nonstructural protein 1 (nsP1) or nsP2 on the infectivity and pathogenesis of Sindbis virus (SINV) have been investigated. We have also confirmed the essential role of E2 glycoprotein, especially the domain of 145-150 (amino acid) aa, in SINV infection through the interaction with cellular heparan sulfate (HS). In addition, we have developed XJ-160 virus-based vector system, including replicon vector, defective helper (DH) plasmids and the packaging cell lines (PCLs). Here we provide an update of main development in the field concerned with XJ-160 virus.
作为一种辛德毕斯样病毒（SINLV），XJ-160 病毒于 1990 年从中国新疆收集的一组按蚊样本中分离出来。重组质粒 pBR-XJ160 是 XJ-160 病毒的全长感染性 cDNA 克隆，可通过体外转录和转染获得拯救病毒 BR-XJ160。在 BHK-21 细胞中培养的 BR-XJ160 病毒在生物学特性上与 XJ-160 病毒没有区别，包括其蚀斑形态、生长动力学和幼鼠神经毒力。在 pBR-XJ160 的基础上，研究了非结构蛋白 1（nsP1）或 nsP2 内的替换对辛德毕斯病毒（SINV）的感染力和发病机制的影响。我们还通过 E2 糖蛋白与细胞肝素硫酸盐（HS）的相互作用，证实了 E2 糖蛋白在 SINV 感染中的重要作用，特别是 145-150（氨基酸）aa 域的作用。此外，我们还开发了基于 XJ-160 病毒的载体系统，包括复制子载体、缺陷型辅助（DH）质粒和包装细胞系（PCL）。在此，我们提供了 XJ-160 病毒相关领域主要发展情况的最新信息。
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