评估在 8 字形离心逆流色谱中蛋白质分离的性能。
Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography.
机构信息
Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1762, USA.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Dec 15;879(32):3802-5. doi: 10.1016/j.jchromb.2011.10.019. Epub 2011 Oct 25.
The performance of protein separation using the figure-8 column configuration in centrifugal counter-current chromatography was investigated under various flow rates and revolution speeds. The separation was performed with a two-phase solvent system composed of polyethylene glycol 1000/potassium phosphate each at 12.5% (w/w) in water and with lysozyme and myoglobin as test samples. In order to improve tracing of the elution curve, a hollow fiber membrane dialyzer was inserted at the inlet of the UV detector. The results showed that the retention of stationary phase (Sf) and resolution (Rs) increased with decreased flow rate and increased revolution speed. The highest Rs of approximately 1 was obtained at a flow rate of 0.01 mL/min under a revolution speed of 1200 rpm with a 3.4 mL capacity column.
采用八形柱结构的离心逆流色谱在不同流速和转速下的蛋白质分离性能进行了研究。该分离采用由聚乙二醇 1000/磷酸钾组成的两相溶剂体系，在水中的浓度均为 12.5%（w/w），以溶菌酶和肌红蛋白为测试样品。为了提高洗脱曲线的跟踪效果，在紫外检测器的入口处插入了中空纤维膜透析器。结果表明，固定相（Sf）保留率和分辨率（Rs）随流速降低和转速增加而增加。在 3.4 mL 容量柱下，在转速为 1200 rpm 时，流速为 0.01 mL/min 时，获得了约 1 的最高 Rs。
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